Target enrichment from a DNA mixture by oligoribonucleotide interference-PCR (ORNi-PCR)
نویسندگان
چکیده
منابع مشابه
Oligoribonucleotide (ORN) Interference-PCR (ORNi-PCR): A Simple Method for Suppressing PCR Amplification of Specific DNA Sequences Using ORNs
Polymerase chain reaction (PCR) amplification of multiple templates using common primers is used in a wide variety of molecular biological techniques. However, abundant templates sometimes obscure the amplification of minor species containing the same primer sequences. To overcome this challenge, we used oligoribonucleotides (ORNs) to inhibit amplification of undesired template sequences withou...
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ژورنال
عنوان ژورنال: Biology Methods and Protocols
سال: 2019
ISSN: 2396-8923
DOI: 10.1093/biomethods/bpz009